Biology Topic Practice

Benedict's Test for Sugars

The Benedict's test is the standard GCSE method for identifying reducing sugars like glucose in food samples.

Why must you use a hot water bath rather than a direct flame?

What is Benedict's Test for Sugars?

"Benedict's test is a chemical test for the presence of reducing sugars which requires heating."

Question 1

What colour change shows starch is present when iodine solution is added?

Question 2

Which result would show protein is present?

Question 3

A food sample gives a brick-red precipitate with Benedict’s solution after heating. What does this show?

Question 4

Which observation is a positive result for the emulsion test for lipids?

Test Protocol

Add Benedict's solution and heat the sample in a water bath set to 80°C.

A range of colours from green to brick-red reflects the concentration of sugar present.

Exam Tip

Make sure to use an excess of Benedict's solution to ensure all sugar reacts.

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