Biology Topic Practice

Biuret Test for Proteins

The Biuret test uses copper sulphate and sodium hydroxide to detect protein.

What colour change indicates a positive result?

What is Biuret Test for Proteins?

"The biuret test is a chemical test used for detecting the presence of peptide bonds (proteins)."

Question 1

What colour change shows starch is present when iodine solution is added?

Question 2

Which result would show protein is present?

Question 3

A food sample gives a brick-red precipitate with Benedict’s solution after heating. What does this show?

Question 4

Which observation is a positive result for the emulsion test for lipids?

Testing for Proteins

Add Biuret reagent (or NaOH then CuSO4) to the sample.

Shake gently and wait for a few minutes.

Positive result: colour change from blue to purple/lilac.

Exam Tip

Biuret reagent is corrosive; handle with care and wear eye protection.

Want to improve faster?

The fastest way to improve your grade is to practise exam-style questions, identify your gaps, and focus only on what you don't know.

Start practising this topic
Instantly marked • No sign-up

Related GCSE Biology Questions

View all GCSE Biology topics

Verified for 2024/25 Exams

Aligned to current UK Science Specifications

LO

Little & Often Content Team

Science Education Specialists