Culturing Microorganisms Practical
This required practical involves growing bacteria on agar plates to test the effectiveness of antibiotics.
Why do we incubate plates at 25°C in schools?
What is Culturing Microorganisms Practical?
"Culturing microorganisms is the process of growing bacteria or other microbes in a controlled environment like a petri dish."
How do bacteria usually reproduce?
Why must Petri dishes and culture media be sterilised before use?
Why are cultures in school laboratories usually incubated at about 25°C rather than 37°C?
Aseptic Technique
Sterilise equipment (e.g. heating inoculating loop) to prevent contamination.
Secure the lid with tape (not all the way around) to allow oxygen.
Storage at 25°C prevents the growth of dangerous human pathogens.
Aseptic technique means "without germs"; it ensures you only grow the bacteria you intended.
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