Biology Topic Practice
pH and Amylase Practical
This required practical investigates how the rate of starch breakdown by amylase is affected by pH.
What reagent do we use to test for the disappearance of starch?
What is pH and Amylase Practical?
"The pH and amylase practical is an experiment to determine the optimum pH for the enzyme amylase."
Question 1
Which nutrient is broken down by amylase?
Question 2
Which nutrient is broken down by amylase?
Question 3
Where is amylase produced?
Practical Method
Mix amylase, starch, and a buffer solution of a specific pH.
Every 30 seconds, add a drop to iodine on a spotting tile.
Stop when the iodine remains orange (no starch left).
Exam Tip
The independent variable is the pH; the dependent variable is the time taken for starch to disappear.
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