Biology Topic Practice

pH and Amylase Practical

This required practical investigates how the rate of starch breakdown by amylase is affected by pH.

What reagent do we use to test for the disappearance of starch?

What is pH and Amylase Practical?

"The pH and amylase practical is an experiment to determine the optimum pH for the enzyme amylase."

Question 1

Which nutrient is broken down by amylase?

Question 2

Which term correctly completes this GCSE Biology equation or calculation statement? ____ change = (final − initial) ÷ initial × 100

Question 3

Which answer best completes the statement? ____ quadrat sampling uses random numbers to choose squares before placing quadrats

Question 4

Which nutrient is broken down by amylase?

Mix amylase, starch, and a buffer solution of a specific pH.

Every 30 seconds, add a drop to iodine on a spotting tile.

Stop when the iodine remains orange (no starch left).

Exam Tip

The independent variable is the pH; the dependent variable is the time taken for starch to disappear.

The fastest way to improve your grade is to practise exam-style questions, identify your gaps, and focus only on what you don't know.

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